Determination of Sensitivity Level of Hypocotyl Explants to Paromomycin Antibiotic and Optimization of Rooting in Transgenic Medicinal Plant Papaver somniferum

  • Parvin Norouzi
  • Ahamad Ismaili
  • Farhad Nazarian FirouzAbadi
  • Ali Mohammad Latifi

Abstract

Poppy (Papaver somniferum) is the most important commercial source of analgesic drugs and of compounds related to them. Because of the excellent medicinal prop- erties of poppy, finding genotypes with changed alkaloid content through breeding or by producing transgenic plants in tissue culture has attracted great interest. The present research was conducted to optimize rooting in somatic embryos of trans- genic poppy plants. Sensitivity of hypocotyl explants to the antibiotic paromomycin was first determined in kill-curve experiments. Transformation of the hypocotyl explants was carried out using the GV3101 strain of Agrobacterium tumefaciens
having the vector with the NPT II gene. The explants were then transferred to MS- based medium containing the 2, 4-D plant growth regulator (PGR) at 1 mg/L, 10 mM MES, and paromomycinat 15 mg/L. After the somatic embryos were pro- duced, a factorial experiment was conducted in the rooting stage with two factors including medium (MS and 1/2 MS) and PGR (500 μM IBA PGR and no PGR). Also, confirmation of derived transgenic plantlets was confirmed by PCR tech- nique. Results of ANOVA and comparison of the means indicated the highest root- ing percentage happened in the 1/2 MS medium containing the IBA.

Published
2018-03-13
How to Cite
NOROUZI, Parvin et al. Determination of Sensitivity Level of Hypocotyl Explants to Paromomycin Antibiotic and Optimization of Rooting in Transgenic Medicinal Plant Papaver somniferum. Journal of Applied Biotechnology Reports, [S.l.], v. 4, n. 3, p. 651-655, mar. 2018. ISSN 2423-5784. Available at: <https://journals.bmsu.ac.ir/jabr/index.php/jabr/article/view/211>. Date accessed: 22 june 2018.
Section
Original/Research Articles

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.