Isolating Two Native Extreme Halophilic Bacterial Strains Producing Bacteriorhodopsin Protein from Aran-Bidgol Lake
AbstractBacteriorhodopsin operates as a light/proton transfer pump which converts the light energy into a proton gradient. Energy stored in the proton gradient can be used in a variety of ways. Bacteriorhodopsin producing sources are some Halobacterium species such as Halobacterium sodomense and Halobacterium salinarum which continue to grow in harsh and salt-saturated conditions. Two types of red pigment (IRLS.1) and orange pigment (IRLS.2) producing strains were isolated in order to isolate bacteriorhodopsin producing strains from Aran-Bidgol Lake. Spectroscopy reviews and the results of SDS-Page of membrane proteins of two isolated strains and Iranian native Halobacterium salinarum showed that there is bacteriorhodopsin protein in the sample. Spectroscopic studies showed that Halobacterium salinarum produces the maximum amount, then IRLS.1 and finallyIRLS.2 with the lowest amount of bacteriorhodopsin. The results of biochemical and molecular identification based onthe16srRNA of both mentioned strains indicated their highest similarity with Natrinema sp. XA3-1 and Archaeon RC34, respectively. In this study, the presence of bacteriorhodopsin protein in Iranian native strains was examined for the first time and was isolated purely from Halobacterium salinarum membrane by gel filtration chromatography that given the widespread use of bacteriorhodopsin protein, it will be so effective.
2. Lozier, R.H., Bogomolni, R.A., Stoeckenius, W., Bacteriorho-dopsin: a light-driven proton pump in Halobacterium Halobium. J Biophys, 1975, Vol. 15(9), pp. 955-962.
3. Shamansky, L.M., Luong, K.M., Han, D., Chronister, EL., Photoinduced kinetics of bacteriorhodopsin in a dried xerogel glass. J Biosens Bioelectron, 2002, Vol. 17 (3), pp. 227-231.
4. Hendler, R.W., Dracheva, S., Importance of lipids for bacteri-orhodopsin structure, photocycle, and function. J Biochem, 2001, Vol. 66 (11), pp. 1311-1314.
5. Henderson. R., Baldwin, J.M., Ceska, T.A., Zemlin, F., Beck-mann, E., Downing, K.H., Model for the structure of bacteriorho-dopsin based on high-resolution electron cryo-microscopy. J Mol Biol, 1990, Vol. 213(4), pp. 899-929.
6. Hirai, T., Subramaniam, S., Protein conformational changes in the bacteriorhodopsin photocycle: comparison of findings from electron and X-ray crystallographic analyses. J PLoS One, 2009, Vol. 4(6), pp.e5769.
7. Dunn, R., McCoy, J., Simsek, M., Majumdar, A., Chang, S.H., Rajbhandary. U.L., Khorana, H.G., The bacteriorhodopsin gene. J Proc Natl Acad Sci USA, 1981, Vol. 78(11), pp.6744-6748.
8. Khorana, H.G., Gerber, G.E., Herlihy, W.C., Gray, C.P., An-deregg, R.J., Nihei, K., Biemann, K., Amino acid sequence of bacteriorhodopsin. J Proc Natl Acad Sci USA, 1979, Vol. 76 (10), pp.5046-5050.
9. Oren, A., Halobacterium sodomense sp. nov. A Dead Sea Ha-lobacterium with an Extremely High Magnesium Requirement. International journal of systematic bacteriology, 1983, Vol. 33(2), pp.381-386.
10. Pandey, P.C., Upadhyay, B.C., Pandey, C.M.D., Pathak, H.C., Dependence of M, N and O states decay kinetics of D96N bacteri-orhodopsin on amine and amino compounds and its application in chemical sensing. J Sensors and Actuators B-Chemical, 1998, Vol. 46, pp.80-86.
11. Hassanshahian, M., Mohamadian, J., Isolation and characteri-zation of Halobacterium salinarum from saline lakes in Iran. Jun-dishapur J Microbiol, 2011, Vol. 4(5), pp. S59-S65.
12. Margesin, R., Schinner, F., Potential of halotolerant and halo-philic microorganisms for biotechnology. J Extremophiles, 2001, Vol. 5(2), pp. 73-83.
13. Wise, K.J., Gillespie, N.B., Stuart, J.A., Krebs, M.P., Birge, RR., Optimization of bacteriorhodopsin for bioelectronic devices. J Trends Biotechnol, 2002, Vol. 20(9), pp. 387-394.
14. Geibel, S., Lörinczi, E., Bamberg, E., Friedrich, T., The volt-age-dependent proton pumping in bacteriorhodopsin is character-ized by optoelectric behavior. Biophys J, 2001, Vol. 81(4), pp. 2059-2068.
15. Huh, Y., Jeong, C., Chang, H., Lee, S., Hong, W., Park, T., Rapid separation of bacteriorhodopsin using a laminar-flow extrac-tion system in a microfluidic device. J Biomicrofluidics, 2010, Vol. 4(1), pp. 14103
16. Lee, S.Y., Chang, H.N., Um, Y.S., Hong, S.H., Bacteriorho-dopsin production by cell recycleculture of Halobacterium halobi-um. J Biotechnol Let, 1998, Vol. 20(8), pp.763-765
17. Zeng, C., Zhu, J.C., Liu, Y., Yang, Y., Zhu, JY., Huang, Y.P., Shen, P., Investigation of the influence of NaCl concentration on Halobacterium salinarum growth - Microcalorimetry and transmis-sion electron microscopy. J Therm Anal Calorim, 2006, Vol.8 4(3), pp. 625-630.
18. Ghasemi, M.F., Shodjai-Arani, A., Moazami, N., Optimization of bacteriorhodopsin production by Halobacterium salinarium PTCC 1685. J Process Biochem, 2008, Vol. 43(10), pp. 1077-1082.
Lobanova, K.V., Tashpulatov, Z., Pshenichnov, E.A., Gulyamova, TG., Synthesis of Bacteriorhodopsin by Halobacterium Sodomense K91r. J Chem Nat Comp, 2011, Vol. 47(5), pp.862-863.
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