Diisopropyl-fluorophosphatase as a Catalytic Bioscavenger
Organophosphorus chemicals, used as pesticides and warfare nerve agent, are highly toxic compounds that inhibit acetylcholine esterase enzyme rapidly. A novel effective treatment for nerve gas poising is using of hydrolytic enzymes to degradation of these agents. DFPase from loligo vulgaris is highly stable and robust biocatalyst for the hydrolysis of various chemical warfare agents such as sarin, soman, tabun. Unfortunately, wild-type DFPase prefers less toxic isomers of these agents leading to slower detoxification. Also, due to non-human origin of the enzyme, immunological reactions occur when it injects into body. Engineering of the enzyme for creating of new efficient variants is an interesting research field which leads to occurrence of novel and prominent bioscavengers.
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