Assessment of Third Generation Cephalosporin (Ceftazidime and Ceftriaxone) Resistant Escherichia Coli Strains Isolated from Zahedan Hospitals by Tracing the TEM Gene

  • Somaye Jahani
  • Mehdi Ghamgosha
  • Amin Shakiba
  • Kazem Hassanpour
  • Ramezan Ali Taheri
  • Gholamreza Farnoosh

Abstract

Escherichia coli is  a Gram-negative, facultative  anaerobic, rod-shaped  bacte- rium and member of the Enterobacteriaceae family. E. coli is common in various infections, including hospital-acquired urinary tract infections. Ceftriaxone and ceftazidime are most commonly-used antibiotics to treat infections caused by Ente- robacteriaceae. The purpose of this study was to determine the antimicrobial resis- tance pattern of E. coli strains isolated from patients referred to the selected hospit- als in Zahedan by tracing the blaTEM beta-lactamase gene. Over a 12 month period, 200 clinical samples were examined. Antibiotic susceptibility was deter- mined by disk diffusion test and microdilution method and the presence of bla TEM gene was evaluated by PCR. 130 isolates were potentially extended-spectrum beta- lactamase-producing and 72 isolates contained the TEM gene. The results of the present study indicate a high rate of antibiotic resistance among E. coli isolates to ceftriaxone and ceftazidime. Therefore, it is recommended to perform antibiogram tests before prescribing antibiotic therapy.


 


 


 


 


 


 


 


Keywords: Ecsherichia coli, Disk Diffusion Test, Extended Spectrum Beta- lactamases (ESBLs)

Published
2017-08-14
How to Cite
JAHANI, Somaye et al. Assessment of Third Generation Cephalosporin (Ceftazidime and Ceftriaxone) Resistant Escherichia Coli Strains Isolated from Zahedan Hospitals by Tracing the TEM Gene. Journal of Applied Biotechnology Reports, [S.l.], v. 4, n. 1, p. 547-552, aug. 2017. ISSN 2423-5784. Available at: <http://journals.bmsu.ac.ir/jabr/index.php/jabr/article/view/163>. Date accessed: 25 sep. 2017.
Section
Original/Research Articles

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