Isolation and Identification of a Keratinolytic Bacillus cereus and Optimization of Keratinase Production

  • Fathollah Ahmadpour National Institute of Genetic Engineering and Bio-technology, Iran, Tehran
  • Bagher Yakhchali National Institute of Genetic Engineering and Bio-technology, Iran, Tehran
  • Masoumeh Sadat Musavi National Institute of Genetic Engineering and Bio-technology, Iran, Tehran

Abstract

A keratin-degrading bacterium was selected from a bacterial collection of the Najm
Biotech Company. Molecular identification indicated that the bacterium is a strain of Bacillus cereus, which can grow and produce keratinase in basal medium containing feather as sole source of carbon and energy. The physicochemical condition (pH, temperature, incubation time, feather concentration) of keratinase production of the isolated B.cereus strain was optimized using response surface methodology (RSM). A maximum keratinase production of 350 U/ml was achieved in 96 h under optimized conditions. 

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Published
2017-06-03
How to Cite
AHMADPOUR, Fathollah; YAKHCHALI, Bagher; MUSAVI, Masoumeh Sadat. Isolation and Identification of a Keratinolytic Bacillus cereus and Optimization of Keratinase Production. Journal of Applied Biotechnology Reports, [S.l.], v. 3, n. 4, p. 507-512, june 2017. ISSN 2423-5784. Available at: <http://journals.bmsu.ac.ir/jabr/index.php/jabr/article/view/143>. Date accessed: 17 oct. 2017.
Section
Original/Research Articles

Keywords

Feathers, Keratinase, Bacillus cereus, Optimization, Response Surface Methodology

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