Screening and Isolation of Collagenase Producing Microorganism from Proteins Waste Found in Himalayan Region

  • Manisha Gautam HPU-Shimla- H.P. India
  • Wamik Azmi

Abstract

Multicellular organisms of the human and animal bodies such as skin, bones, ten- dons, cartilage and blood vessels have collagen as a fibrous element. Collagen is a most representative protein of the leather wastes and collagenase is a proteolytic enzyme. The collagenase is responsible for the degradation of native collagen to small peptide fragments, without affecting the other proteins. Collagenase has widespread applications such as cosmetics, wound healing, diabetic ulcer, arterial ulcers and burns surgery, etc. In the present study, Twenty-seven collagenase- producing microorganisms were identified and isolated from soil/sewage samples  of the fish market and slaughterhouse areas. CS-20 sample was found most effi- cient producer microorganism of collagenase. This sample was identified from Pseudomonas genus and isolated as Pseudomonas species. The parameters such as temperature, substrate, pH, incubation period and inoculum percentage were also optimized to screen the hyper producer strain with maximum collagenase activity. CS-20 isolate (Collagenase producing microorganism) was capable of hydrolyzing other protein substrates such as gelatine and azocoll. Thus, CS-20 isolate is high- efficiency strain for the production of collagenase.


 


 


Keywords: Microorganisms, Proteolytic Enzyme, Collagenase, Pseudomonas Ge- nus

Author Biography

Manisha Gautam, HPU-Shimla- H.P. India
Researcher
Published
2017-08-14
How to Cite
GAUTAM, Manisha; AZMI, Wamik. Screening and Isolation of Collagenase Producing Microorganism from Proteins Waste Found in Himalayan Region. Journal of Applied Biotechnology Reports, [S.l.], v. 4, n. 1, p. 558-565, aug. 2017. ISSN 2423-5784. Available at: <http://journals.bmsu.ac.ir/jabr/index.php/jabr/article/view/139>. Date accessed: 25 sep. 2017.
Section
Original/Research Articles

Keywords

Microorganisms, Proteolytic enzyme, Collagenase, Pseudomonas genus

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