Expression Patterns of Transcription Factors (Zpt2-1, CBF4, bHLH) under Salt Stress in Alfalfa using qPCR

  • Ahmad Ali Shoushi Dezfuli Improvement Institute Department, Safiabad Agricultural and Natural Resource Research Center, AREEO, Dezful, Iran.
  • Sharam Mohammadi Dehcheshmeh2 Professor of department of plant breeding and biotechnology, Faculty of Agriculture, Shahrekord University, PO Box 115, Shahrekord, Iran. Tel: +98 (913) 381 5583.
  • Fariba Rafiei Associate Professor of department of plant breeding and biotechnology, Faculty of Agriculture, Shahrekord University, PO Box 115, Shahrekord, Iran. Tel: +98 (913) 280 3015
  • Behrouz Shiran Professor of department of plant breeding and biotechnology, Faculty of Agriculture, Shahrekord University, PO Box 115, Shahrekord, Iran

Abstract

Researchers need continuously improve their views on mechanisms of salt toler- ance in crop plants in order to overcome their limitations and improve yields under stress conditions including salt stress. One of the most important methods of con- trolling stresses in plants is through making adjustments during the process of gene transcription. Transcription factors adjust the degree of expression in many genes by binding to DNA and are thus very important in salt tolerance of plants. There- fore, the present research studied the role of transcription factors bHLH, CBF4, and Zpt2-1in salt tolerance by investigating their expression patterns under salt stress in the leaf and root tissues of the Yazdi (salt tolerant) and the Diabloverde (sensitive  to salinity) genotypes of Alfalfa. Results showed that short-term salt stress affects on expression patterns of the CBF4, Zpt2-1, and bHLH genes in leaf and root tis- sues of both genotypes. Using qRT-PCR (Real-Time PCR) analysis, it was shown that the transcription factors Zpt2-1 and CBF4 in the salt tolerant genotype (Yazdi) were expressed at a higher level in root tissues. In other words, it seems that higher expression levels of the transcription factors Zpt2-1 and CBF4 were accompanied by greater salt tolerance. This finding can help plant breeders to use these transcrip- tion factors for selecting salt-tolerant genotypes in alfalfa.


Keywords: Medicago sativa, Real-Time PCR, Salt Tolerance

Published
2017-08-14
How to Cite
SHOUSHI DEZFULI, Ahmad Ali et al. Expression Patterns of Transcription Factors (Zpt2-1, CBF4, bHLH) under Salt Stress in Alfalfa using qPCR. Journal of Applied Biotechnology Reports, [S.l.], v. 4, n. 1, p. 541-546, aug. 2017. ISSN 2423-5784. Available at: <http://journals.bmsu.ac.ir/jabr/index.php/jabr/article/view/130>. Date accessed: 25 sep. 2017.
Section
Original/Research Articles

Keywords

Alfalfa, qRT-PCR, salt stress, transcription factors

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.