Enzymatic and Non-Enzymatic Antioxidants of Calamintha officinalis Moench Extracts

  • Fatemeh Shams Moattar Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • Reyhaneh Sariri Khayatzadeh 2. Department of Chemistry, Islamic Azad Univer-sity, Lahijan Branch, Lahijan, Iran
  • Parichehr Yaghmaee Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • Masoud Giahi Department of Chemistry, Islamic Azad University, Lahijan Branch, Lahijan, Iran

Abstract

Calamintha officinalis (COM) is a genus of aromatic herbs, native in north of Iran. Partial purification of aqueous extract from COM was done using ammonium sulfate precipitation followed by dialysis. The total protein content was determined at various purification stages. The highest amount of protein of the extract was found in fraction obtained in 20-40% saturation range. Specific activities of three antioxidant enzymes, peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) were also determined and compared in each stage. The highest activity of POD, CAT and SOD was in fractions 60-80%, 20-40%, and 20-40% respectively. The non-enzymatic antioxidants were evaluated by two different methods i.e. free radical scavenging activity using DPPH and inhibition of lipid peroxidation by the ferric thiocyanate method. It was found that radical scavenging power of COM methanolic extract was 43.99. Furthermore, the inhibition of lipid peroxidation of the extract was decreased every 24 h. Thus, our findings suggested that C.officinalis possess potent antioxidant defense machinery both enzymatic and non enzymatic.

Author Biography

Fatemeh Shams Moattar, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Biology

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Published
2017-06-03
How to Cite
MOATTAR, Fatemeh Shams et al. Enzymatic and Non-Enzymatic Antioxidants of Calamintha officinalis Moench Extracts. Journal of Applied Biotechnology Reports, [S.l.], v. 3, n. 4, p. 489-494, june 2017. ISSN 2423-5784. Available at: <http://journals.bmsu.ac.ir/jabr/index.php/jabr/article/view/129>. Date accessed: 24 aug. 2017.
Section
Original/Research Articles

Keywords

Calamintha officinalis, Antioxidants, POD, CAT, SOD

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